- Catalog number
- FEK0473
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 31 33 29 31 1.41 4.5% Sample 2 195 189 201 195 195 4.24 2.1% Sample 3 532 466 458 478 483 28.89 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 31 33 29 31 1.41 4.5% Sample 1 31 31 33 29 31 1.41 4.5% Sample 2 195 189 201 195 195 4.24 2.1% Sample 2 195 189 201 195 195 4.24 2.1% Sample 3 532 466 458 478 483 28.89 5.9% Sample 3 532 466 458 478 483 28.89 5.9%
- Synonyms
- Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor; Ntf3; Ntf-3
- Gene Name
- NTF3
- Gene ID
- 18205
- UniProt
- P20181
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Y139-T257
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 131 532 31 208 563 Standard deviation 2.29 2.85 29.79 2.85 9.36 33.78 CV (%) 7.4% 4% 5.6% 9.2% 4.5% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 131 532 31 208 563 Standard deviation 2.29 2.85 29.79 2.85 9.36 33.78 CV (%) 7.4% 4% 5.6% 9.2% 4.5% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 131 532 31 208 563 Mean (pg/ml) 31 131 532 31 208 563 Standard deviation 2.29 2.85 29.79 2.85 9.36 33.78 Standard deviation 2.29 2.85 29.79 2.85 9.36 33.78 CV (%) 7.4% 4% 5.6% 9.2% 4.5% 6% CV (%) 7.4% 4% 5.6% 9.2% 4.5% 6%
- Sensitivity
- <2 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 29588 MW
- Specificity
- Natural and recombinant mouse Neurotrophin-3
- Gene Name Synonym
- Neurotrophin-3
- Protein Name
- Neurotrophin-3
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.056 0.097 0.203 0.388 0.691 1.286 1.906 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.056 0.097 0.203 0.388 0.691 1.286 1.906 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.056 0.097 0.203 0.388 0.691 1.286 1.906 O.D. 0.003 0.056 0.097 0.203 0.388 0.691 1.286 1.906
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
