Human CXCL16 PicoKine® Quick ELISA Kit

About this product

Human CXCL16 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CXCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0741

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Sample 3 2351 2384 2122 2446 2325 122.47 5.2%

Synonyms

C-X-C motif chemokine 16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SR-PSOX; Small-inducible cytokine B16; Transmembrane chemokine CXCL16; CXCL16; SCYB16, SRPSOX; UNQ2759/PRO6714

Gene Name

CXCL16

Gene ID

58191

UniProt

Q9H2A7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N49-P137

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

93.7 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 122 725 2351 133 696 2150 Mean (pg/ml) 122 725 2351 133 696 2150 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 Standard deviation 9.15 55.82 185.72 10.24 57.7%8 197.8 CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2% CV (%) 7.5% 7.7% 7.9% 7.7% 8% 9.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

27579 MW

Specificity

Natural and recombinant human CXCL16

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-X-C motif chemokine 16

Protein Name

C-X-C motif chemokine 16

Sample Data

Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046 O.D. 0.096 0.164 0.291 0.433 0.715 1.073 1.593 2.046

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.