- Catalog number
- FEK2039
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 610 690 615 45.06 7.3% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 3 7933 8323 9098 7778 8283 510.72 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 610 690 615 45.06 7.3% Sample 1 590 572 610 690 615 45.06 7.3% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 3 7933 8323 9098 7778 8283 510.72 6.1% Sample 3 7933 8323 9098 7778 8283 510.72 6.1%
- Synonyms
- Angiopoietin-related protein 2; Angiopoietin-like protein 2
- Gene Name
- ANGPTL2
- Gene ID
- 23452
- UniProt
- Q9UKU9
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q23-H493
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6%
- Sensitivity
- <50 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant human ANGPTL2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Angiopoietin like 2
- Protein Name
- Angiopoietin-related protein 2
- Sample Data
- Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
