- Catalog number
- FEK0397
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Sample 3 484 469 488 480 480 7.08 1.4%
- Synonyms
- Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin; IL2
- Gene Name
- IL2
- Gene ID
- 3558
- UniProt
- P60568
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A21-T153
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1%
- Sensitivity
- <1 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 17628 MW
- Specificity
- Natural and recombinant human IL-2
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-2
- Protein Name
- Interleukin-2
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.798
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
