Human MCP-1 PicoKine® Quick ELISA Kit

About this product

Human MCP-1 / CCL2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0441

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 37 35 34 2.23 6.5% Sample 2 159 139 156 158 153 8.15 5.3% Sample 3 477 421 423 456 444 23.46 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 37 35 34 2.23 6.5% Sample 1 31 33 37 35 34 2.23 6.5% Sample 2 159 139 156 158 153 8.15 5.3% Sample 2 159 139 156 158 153 8.15 5.3% Sample 3 477 421 423 456 444 23.46 5.2% Sample 3 477 421 423 456 444 23.46 5.2%

Synonyms

C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2; CCL2; MCP1, SCYA2

Gene Name

CCL2

Gene ID

6347

UniProt

P13500

Reactivity

Human

Cross Reactivity

There is cross-reactivity with human Eotaxin, MCP-3<1%.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-T99

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 159 477 30 148 481 Mean (pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8% CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

11025 MW

Specificity

Natural and recombinant human MCP-1

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

C-C motif chemokine 2

Protein Name

C-C motif chemokine 2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.071 0.154 0.244 0.466 1.052 1.298 1.892 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.071 0.154 0.244 0.466 1.052 1.298 1.892 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.071 0.154 0.244 0.466 1.052 1.298 1.892 O.D. 0.025 0.071 0.154 0.244 0.466 1.052 1.298 1.892

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.