- Catalog number
- FEK1384
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- ADAM metallopeptidase with thrombospondin type 1 motif 1.This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 121 177 174 143 9.85 6.9% Sample 2 409 642 650 377 520 16.31 3.1% Sample 3 1816 2943 2064 2404 2307 179.23 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 121 177 174 143 9.85 6.9% Sample 1 99 121 177 174 143 9.85 6.9% Sample 2 409 642 650 377 520 16.31 3.1% Sample 2 409 642 650 377 520 16.31 3.1% Sample 3 1816 2943 2064 2404 2307 179.23 7.8% Sample 3 1816 2943 2064 2404 2307 179.23 7.8%
- Synonyms
- Adamts1; A disintegrin and metalloproteinase with thrombospondin motifs 1; ADAM-TS 1; ADAM-TS1; ADAMTS-1; EC 3.4.24.-
- Gene Name
- ADAMTS1
- Gene ID
- 11504
- UniProt
- P97857
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: F254-C725
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 93.8 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 409 1816 147 471 1786 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 409 1816 147 471 1786 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 99 409 1816 147 471 1786 Mean (pg/ml) 99 409 1816 147 471 1786 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 Standard deviation 7.9 13.78 140.74 7.2 22.09 140.92 CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9% CV (%) 8.0% 3.4% 7.8% 4.9% 4.7% 7.9%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 106 kDa
- Specificity
- Natural and recombinant mouse ADAMTS1
- Anticoagulant
- Heparin
- Gene Name Synonym
- ADAM metallopeptidase with thrombospondin type 1 motif 1
- Protein Name
- A disintegrin and metalloproteinase with thrombospondin motifs 1
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000 O.D. 0.018 0.080 0.168 0.296 0.602 1.001 1.555 2.000
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
