- Catalog number
- EK0585
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 208 192 307 238 8.92 3.8% Sample 2 898 818 924 824 866 56.20 6.5% Sample 3 4739 3374 2777 4604 3874 98.00 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 208 192 307 238 8.92 3.8% Sample 1 244 208 192 307 238 8.92 3.8% Sample 2 898 818 924 824 866 56.20 6.5% Sample 2 898 818 924 824 866 56.20 6.5% Sample 3 4739 3374 2777 4604 3874 98.00 2.5% Sample 3 4739 3374 2777 4604 3874 98.00 2.5%
- Synonyms
- LT, LT-[a], LT-alpha, LT[a], LTalpha, Ltx, TNF-beta, TNFSF1, Tnfb, Tnfsf1b, Tnlg1e, hlb382
- Gene Name
- Lta
- Gene ID
- 16992
- UniProt
- P09225
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L34-L202
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA or citrate) .
- Detection Range
- 156pg/mL-10000pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 898 4739 158 1201 3122 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 898 4739 158 1201 3122 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 244 898 4739 158 1201 3122 Mean (pg/ml) 244 898 4739 158 1201 3122 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 Standard deviation 13.91/td> 19.40 356.85 6.23 49.36 105.52 CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4% CV (%) 5.7% 2.2%% 7.5% 3.9% 4.1% 3.4%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22 kDa
- Specificity
- Natural and recombinant mouse Lta
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Lymphotoxin A
- Protein Name
- Lymphotoxin-alpha
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373 O.D. 0.053 0.112 0.156 0.227 0.374 0.813 1.47 2.373
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
