- Catalog number
- FEK0389
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL) -1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 15 15 13 14 0.82 5.8% Sample 2 52 48 50 48 49 1.65 3.3% Sample 3 99 98 112 102 102 5.53 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 15 15 13 14 0.82 5.8% Sample 1 14 15 15 13 14 0.82 5.8% Sample 2 52 48 50 48 49 1.65 3.3% Sample 2 52 48 50 48 49 1.65 3.3% Sample 3 99 98 112 102 102 5.53 5.4% Sample 3 99 98 112 102 102 5.53 5.4%
- Synonyms
- Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1
- Gene Name
- IL1A
- Gene ID
- 3552
- UniProt
- P01583
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S113-A271
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 4.7 pg/mL - 300 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 14 52 99 15 49 103 Mean (pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9% CV (%) 7% 4.8% 4.4% 7.1% 4.9% 5.9%
- Sensitivity
- <0.5 pg/mL
- Function
- Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 31 kDa
- Specificity
- Natural and recombinant human IL-1 alpha
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-1 alpha
- Subcellular Location
- Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Sequence Similarities
- Belongs to the IL-1 family.
- Protein Name
- Interleukin-1 alpha
- Sample Data
- Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.033
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
