Anti-YTHDF1 Antibody Picoband®

About this product

Boster Bio Anti-YTHDF1 Antibody Picoband® catalog # A09853-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09853-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Enables N6-methyladenosine-containing RNA reader activity and ribosome binding activity. Involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Located in P-body and cytoplasmic stress granule. Implicated in colorectal cancer.

Synonyms

YTHDF1; C20orf21; YTH domain-containing family protein 1; Dermatomyositis associated with cancer putative autoantigen 1; DACA-1

Gene Name

YTHDF1

Gene ID

54915

UniProt

Q9BYJ9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human YTHDF1. Human YTHDF1 shares 95.2% amino acid (aa) sequence identity with mouse YTHDF1.

Clonality

Polyclonal

Applications

WB, IHC, ICC/IF, Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Han, D., Liu, J., Chen, C., Dong, L., Liu, Y., Chang, R., Huang, X., Liu, Y., Wang, J., Dougherty, U., Bissonnette, M. B., Shen, B., Weichselbaum, R. R., Xu, MM., He, C. Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells. Nature 566: 270-274, 2019. Note: Erratum: Nature 568: E3, 2019. Electronic Article. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 8/19/2015. 3. Li, A., Chen, Y.-S., Ping, X.-L., Yang, X., Xiao, W., Yang, Y., Sun, H.-Y., Zhu, Q., Baidya, P., Wang, X., Bhattarai, D. P., Zhao, Y.-L., Sun, B.-F., Yang, Y.-G. Cytoplasmic m6A reader YTHDF3 promotes mRNA translation. (Letter) Cell Res. 27: 444-447, 2017.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

61 kDa

Observed Molecular Weight

65 kDa

Applications Notes

6

Gene Name Synonym

YTH N6-methyladenosine RNA binding protein F1

Protein Name

YTH domain-containing family protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.