Anti-SHMT2 Antibody Picoband®

About this product

Boster Bio Anti-SHMT2 Antibody Picoband® catalog # A03233-1. Tested in WB, IHC, IF, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03233-1-carrier-free

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.

Synonyms

SHMT2; Serine hydroxymethyltransferase, mitochondrial; SHMT; EC 2.1.2.1; Glycine hydroxymethyltransferase; Serine methylase

Gene Name

SHMT2

Gene ID

6472

UniProt

P34897

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SHMT2 recombinant protein (Position: A9-H504) . Human SHMT2 shares 95.1% amino acid (aa) sequence identity with mouse SHMT2.

Clonality

Polyclonal

Applications

WB, IHC, IF, ICC/IF, IP, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Bogenhagen, D. F., Rousseau, D., Burke, S. The layered structure of human mitochondrial DNA nucleoids. J. Biol. Chem. 283: 3665-3675, 2008. 2. Cao, J., Sun, L., Aramsangtienchai, P., Spiegelman, N. A., Zhang, X., Huang, W., Seto, E., Lin, H. HDAC11 regulates type I interferon signaling through defatty-acylation of SHMT2. Proc. Nat. Acad. Sci. 116: 5487-5492, 2019. 3. Garcia-Cazorla, A., Verdura, E., Julia-Palacios, N., Anderson, E. N., Goicoechea, L., Planas-Serra, L., Tsogtbaatar, E., Dsouza, N. R., Schluter, A., Urreizti, R. Tarnowski, J. M., Gavrilova, R. H., and 15 others. Impairment of the mitochondrial one-carbon metabolism enzyme SHMT2 causes a novel brain and heart developmental syndrome. Acta Neuropath. 140: 971-975, 2020.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

56 kDa

Observed Molecular Weight

56 kDa

Applications Notes

6

Gene Name Synonym

Serine hydroxymethyltransferase 2

Protein Name

Serine hydroxymethyltransferase, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.