Anti-PIF1 Antibody Picoband®

About this product

Boster Bio Anti-PIF1 Antibody Picoband® catalog # A02315-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02315-1-carrier-free

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a DNA-dependent adenosine triphosphate (ATP) -metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus.

Synonyms

PIF1; C15orf20; ATP-dependent DNA helicase PIF1; EC 3.6.4.12; DNA repair and recombination helicase PIF1; PIF1/RRM3 DNA helicase-like protein

Gene Name

PIF1

Gene ID

80119

UniProt

Q9H611

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PIF1 recombinant protein (Position: Q31-R618) . Human PIF1 shares 87.6% and 87.8% amino acid (aa) sequence identity with mouse and rat PIF1, respectively.

Clonality

Polyclonal

Applications

WB, IHC, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Mateyak, M. K., Zakian, V. A. Human PIF helicase is cell cycle regulated and associates with telomerase. Cell Cycle 5: 2796-2804, 2006. 2. Paeschke, K., Bochman, M. L., Garcia, P. D., Cejka, P., Friedman, K. L., Kowalczykowski, S. C., Zakian, V. A. Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature 497: 458-462, 2013. 3. Zhang, D.-H., Zhou, B., Huang, Y., Xu, L.-X., Zhou, J.-Q. The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity. Nucleic Acids Res. 34: 1393-1404, 2006.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

77 kDa

Observed Molecular Weight

77 kDa

Applications Notes

6

Gene Name Synonym

PIF1 5'-to-3' DNA helicase

Protein Name

ATP-dependent DNA helicase PIF1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.