Anti-POLD1 Antibody Picoband®

About this product

Boster Bio Anti-POLD1 Antibody Picoband® catalog # A03720-2. Tested in WB, IHC, ICC/IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03720-2-carrier-free

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.

Synonyms

POLD1; POLD; DNA polymerase delta catalytic subunit; EC 2.7.7.7; 3'-5' exodeoxyribonuclease; EC 3.1.11.-; DNA polymerase subunit delta p125

Gene Name

POLD1

Gene ID

5424

UniProt

P28340

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human POLD1 recombinant protein (Position: D25-D1068) . Human POLD1 shares 89.5% and 90.2% amino acid (aa) sequence identity with mouse and rat POLD1, respectively.

Clonality

Polyclonal

Applications

WB, IHC, ICC/IF, IP, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Bellido, F., Pineda, M., Aiza, G., Valdes-Mas, R., Navarro, M., Puente, D. A., Pons, T., Gonzalez, S., Iglesias, S., Darder, E., Pinol, V., Soto, J. L., Valencia, A., Blanco, I., Urioste, M., Brunet, J., Lazaro, C., Capella, G., Puente, X. S., Valle, L. POLE and POLD1 mutations in 529 kindred with familial colorectal cancer and/or polyposis: review of reported cases and recommendations for genetic testing and surveillance. Genet. Med. 18: 325-332, 2016. 2. Chang, L.-S., Zhao, L., Zhu, L., Chen, M.-L., Lee, M. Y. W. T. Structure of the gene for the catalytic subunit of human DNA polymerase delta (POLD1) . Genomics 28: 411-419, 1995. 3. Chung, D. W., Zhang, J., Tan, C.-K., Davie, E. W., So, A. G., Downey, K. M. Primary structure of the catalytic subunit of human DNA polymerase delta and chromosomal location of the gene. Proc. Nat. Acad. Sci. 88: 11197-11201, 1991.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

124 kDa

Observed Molecular Weight

124 kDa

Gene Name Synonym

DNA polymerase delta 1, catalytic subunit

Protein Name

DNA polymerase delta catalytic subunit

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.