Anti-POLG2 Antibody Picoband®

About this product

Boster Bio Anti-POLG2 Antibody Picoband® catalog # A06012-3. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06012-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.

Synonyms

POLG2; MTPOLB; DNA polymerase subunit gamma-2, mitochondrial; DNA polymerase gamma accessory 55 kDa subunit; p55; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-beta

Gene Name

POLG2

Gene ID

11232

UniProt

Q9UHN1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human POLG2 recombinant protein (Position: Q29-E434) . Human POLG2 shares 78.2% amino acid (aa) sequence identity with mouse POLG2.

Clonality

Polyclonal

Applications

WB, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Carrodeguas, J. A., Bogenhagen, D. F. Protein sequences conserved in prokaryotic aminoacyl-tRNA synthetases are important for the activity of the processivity factor of human mitochondrial DNA polymerase. Nucleic Acids Res. 28: 1237-1244, 2000. 2. Carrodeguas, J. A., Theis, K., Bogenhagen, D. F., Kisker, C. Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol-gamma-B, functions as a homodimer. Molec. Cell 7: 43-54, 2001. 3. Dosekova, P., Dubiel, A., Karlowicz, A., Zietkiewicz, S., Rydzanicz, M., Habalova, V., Pienkowski, V. M., Skirkova, M., Han, V., Mosejova, A., Gdovinova, Z., Kaliszewska, M., Tonska, K., Szymanski, M. R., Skorvanek, M., Ploski, R. Whole exome sequencing identifies a homozygous POLG2 missense variant in an adult patient presenting with optic atrophy, movement disorders, premature ovarian failure and mitochondrial DNA depletion. Europ. J. Med. Genet. 63: 103821, 2020.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

55 kDa

Observed Molecular Weight

55 kDa

Gene Name Synonym

DNA polymerase gamma 2, accessory subunit

Protein Name

DNA polymerase subunit gamma-2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.