- Catalog number
- HSEK0375
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 4 5 5 5 5 0.34 7.2% Sample 2 21 19 14 19 18 0.74 4.1% Sample 3 87 67 95 60 77 3.56 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 4 5 5 5 5 0.34 7.2% Sample 1 4 5 5 5 5 0.34 7.2% Sample 2 21 19 14 19 18 0.74 4.1% Sample 2 21 19 14 19 18 0.74 4.1% Sample 3 87 67 95 60 77 3.56 4.6% Sample 3 87 67 95 60 77 3.56 4.6%
- Synonyms
- Interferon gamma; IFN-gamma; Ifng
- Gene Name
- IFNG
- Gene ID
- 15978
- UniProt
- P01580
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: H23-C155
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Released primarily from activated T lymphocytes.
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Immunology, Innate Immunity, Interferons, Signaling Pathways, Stem Cells, TGF Beta, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 3.12-200pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 4 21 87 5 20 58 Standard deviation 0.31 1.55 3.10 0.35 1.35 1.62 CV (%) 7.8% 7.4% 3.6% 6.9% 6.8% 2.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 4 21 87 5 20 58 Standard deviation 0.31 1.55 3.10 0.35 1.35 1.62 CV (%) 7.8% 7.4% 3.6% 6.9% 6.8% 2.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 4 21 87 5 20 58 Mean (pg/ml) 4 21 87 5 20 58 Standard deviation 0.31 1.55 3.10 0.35 1.35 1.62 Standard deviation 0.31 1.55 3.10 0.35 1.35 1.62 CV (%) 7.8% 7.4% 3.6% 6.9% 6.8% 2.8% CV (%) 7.8% 7.4% 3.6% 6.9% 6.8% 2.8%
- Sensitivity
- <0.3 pg/mL
- Function
- Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse IFN gamma
- Gene Name Synonym
- Interferon gamma
- Subcellular Location
- Secreted.
- Protein Name
- Interferon gamma
- Sample Data
- Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.058 0.101 0.136 0.218 0.351 0.602 1.131 1.913 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.058 0.101 0.136 0.218 0.351 0.602 1.131 1.913 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.058 0.101 0.136 0.218 0.351 0.602 1.131 1.913 O.D. 0.058 0.101 0.136 0.218 0.351 0.602 1.131 1.913
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
