- Catalog number
- EK1229
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 156 109 119 127 5.79 4.6% Sample 2 610 433 733 546 581 31.99 5.5% Sample 3 2666 2285 2045 2561 2389 89.60 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 156 109 119 127 5.79 4.6% Sample 1 124 156 109 119 127 5.79 4.6% Sample 2 610 433 733 546 581 31.99 5.5% Sample 2 610 433 733 546 581 31.99 5.5% Sample 3 2666 2285 2045 2561 2389 89.60 3.8% Sample 3 2666 2285 2045 2561 2389 89.60 3.8%
- Synonyms
- Chymase;3.4.21.39; Alpha-chymase; Mast cell protease I; CMA1; CYH, CYM
- Gene Name
- CMA1
- Gene ID
- 1215
- UniProt
- P23946
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin)
- Detection Range
- 93.8-6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 610 2666 119 449 2731 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 610 2666 119 449 2731 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 124 610 2666 119 449 2731 Mean (pg/ml) 124 610 2666 119 449 2731 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2% CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2%
- Sensitivity
- <15 pg/mL
- Function
- Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27325 MW
- Specificity
- Natural and recombinant human Chymase
- Anticoagulant
- Heparin
- Gene Name Synonym
- Chymase
- Subcellular Location
- Secreted. Cytoplasmic granule. Mast cell granules.
- Sequence Similarities
- Belongs to the peptidase S1 family. Granzyme subfamily.
- Protein Name
- Chymase
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079
