Human IL13RA2 ELISA Kit PicoKine®

About this product

Human IL13RA2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL13RA2 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2296

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-13 receptor subunit alpha-2 (IL-13Rα2), also known as CD213A2 (cluster of differentiation 213A2), is a membrane bound protein that in humans is encoded by the IL13RA2 gene. The protein encoded by this gene is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.02 2.0% Sample 2 2 2 2 2 2 0.09 4.4% Sample 3 5 6 7 6 6 0.18 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.02 2.0% Sample 1 1 1 1 1 1 0.02 2.0% Sample 2 2 2 2 2 2 0.09 4.4% Sample 2 2 2 2 2 2 0.09 4.4% Sample 3 5 6 7 6 6 0.18 3.0% Sample 3 5 6 7 6 6 0.18 3.0%

Synonyms

IL-13 receptor subunit alpha-2; IL-13R subunit alpha-2; IL-13R-alpha-2; IL-13RA2; Interleukin-13-binding protein; CD213a2

Gene Name

IL13RA2

Gene ID

3598

UniProt

Q14627

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

0.3-20 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 5 1 2 7 Standard deviation 0.04 0.15 0.31 0.07 0.06 0.53 CV (%) 3.5% 7.3% 6.3% 6.6% 3.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 5 1 2 7 Standard deviation 0.04 0.15 0.31 0.07 0.06 0.53 CV (%) 3.5% 7.3% 6.3% 6.6% 3.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 5 1 2 7 Mean (pg/ml) 1 2 5 1 2 7 Standard deviation 0.04 0.15 0.31 0.07 0.06 0.53 Standard deviation 0.04 0.15 0.31 0.07 0.06 0.53 CV (%) 3.5% 7.3% 6.3% 6.6% 3.1% 7.5% CV (%) 3.5% 7.3% 6.3% 6.6% 3.1% 7.5%

Sensitivity

<50 pg/mL

Function

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

44176 MW

Specificity

Natural and recombinant human IL13RA2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

IL13RA2

Subcellular Location

Cell membrane 1 publication; Single-pass type I membrane protein

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein Name

Interleukin-13 receptor subunit alpha-2

Sample Data

Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.033 0.099 0.157 0.269 0.459 0.829 1.391 2.03 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.033 0.099 0.157 0.269 0.459 0.829 1.391 2.03 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.033 0.099 0.157 0.269 0.459 0.829 1.391 2.03 O.D. 0.033 0.099 0.157 0.269 0.459 0.829 1.391 2.03