- Catalog number
- EK2302
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) also known as CD66d (Cluster of Differentiation 66d), is a member of the carcinoembryonic antigen (CEA) gene family. This gene encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein s phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.05 5.1% Sample 2 2 2 2 2 2 0.1 5.2% Sample 3 8 6 7 5 7 0.15 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.05 5.1% Sample 1 1 1 1 1 1 0.05 5.1% Sample 2 2 2 2 2 2 0.1 5.2% Sample 2 2 2 2 2 2 0.1 5.2% Sample 3 8 6 7 5 7 0.15 2.4% Sample 3 8 6 7 5 7 0.15 2.4%
- Synonyms
- Carcinoembryonic antigen CGM1; Carcinoembryonic antigen-related cell adhesion molecule 3 (CEA cell adhesion molecule 3
- Gene Name
- CEACAM3
- Gene ID
- 1084
- UniProt
- P40198
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- CGM1a, the predominant CGM1 transcript, is granulocyte-specific. Not detected out of the granulocytic lineage, such as monocytes, lymphocytes, spleen, testis, colon, brain, liver, pancreas, thymus, ovary, placenta, skeletal muscle, prostate, small intestine, heart, lung and kidney.
- Applications
- ELISA
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 0.3-20 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 8 1 2 8 Standard deviation 0.05 0.12 0.30 0.05 0.13 0.36 CV (%) 4.7% 6.2% 3.7% 4.5% 6.5% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 8 1 2 8 Standard deviation 0.05 0.12 0.30 0.05 0.13 0.36 CV (%) 4.7% 6.2% 3.7% 4.5% 6.5% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 8 1 2 8 Mean (pg/ml) 1 2 8 1 2 8 Standard deviation 0.05 0.12 0.30 0.05 0.13 0.36 Standard deviation 0.05 0.12 0.30 0.05 0.13 0.36 CV (%) 4.7% 6.2% 3.7% 4.5% 6.5% 4.5% CV (%) 4.7% 6.2% 3.7% 4.5% 6.5% 4.5%
- Sensitivity
- <50 pg/mL
- Function
- Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27091 MW
- Specificity
- Natural and recombinant human CEACAM-6
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CEACAM3
- Subcellular Location
- Membrane ; Single-pass type I membrane protein Philippe Le Mercier
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. CEA family.
- Protein Name
- Cell adhesion molecule CEACAM3
- Sample Data
- Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.025 0.121 0.198 0.379 0.614 1.102 1.525 2.032 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.025 0.121 0.198 0.379 0.614 1.102 1.525 2.032 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.025 0.121 0.198 0.379 0.614 1.102 1.525 2.032 O.D. 0.025 0.121 0.198 0.379 0.614 1.102 1.525 2.032
