- Catalog number
- EK2307
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 298 211 187 221 10.68 4.8% Sample 2 1162 1101 1164 905 1083 21.98 2.0% Sample 3 3258 3486 3903 3808 3614 161.17 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 298 211 187 221 10.68 4.8% Sample 1 187 298 211 187 221 10.68 4.8% Sample 2 1162 1101 1164 905 1083 21.98 2.0% Sample 2 1162 1101 1164 905 1083 21.98 2.0% Sample 3 3258 3486 3903 3808 3614 161.17 4.5% Sample 3 3258 3486 3903 3808 3614 161.17 4.5%
- Synonyms
- Collagen alpha-1 (II) chain; Alpha-1 type II collagen; Collagen alpha-1 (II) chain; Chondrocalcin
- Gene Name
- COL2A1
- Gene ID
- 1280
- UniProt
- P02458
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
- Applications
- ELISA
- Field of Research
- Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 156.0-10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 1162 3258 293 1198 4333 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 1162 3258 293 1198 4333 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 187 1162 3258 293 1198 4333 Mean (pg/ml) 187 1162 3258 293 1198 4333 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0% CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0%
- Sensitivity
- <20 pg/mL
- Function
- Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 141785 MW
- Specificity
- Natural and recombinant human COL2A1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Collagen, type II, alpha 1
- Subcellular Location
- Secreted, extracellular space, extracellular matrix.
- Sample Data
- Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258
