Human IL-2R gamma ELISA Kit PicoKine®

About this product

Human IL-2R gamma ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL-2R gamma in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2308

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL2RA (Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene.The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped "sushi-like" domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs.Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 115 67 89 86 4.29 5.0% Sample 2 388 448 329 450 404 31.29 7.8% Sample 3 1796 1667 1242 1271 1494 70.97 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 115 67 89 86 4.29 5.0% Sample 1 73 115 67 89 86 4.29 5.0% Sample 2 388 448 329 450 404 31.29 7.8% Sample 2 388 448 329 450 404 31.29 7.8% Sample 3 1796 1667 1242 1271 1494 70.97 4.8% Sample 3 1796 1667 1242 1271 1494 70.97 4.8%

Synonyms

GTP-binding nuclear protein Ran; Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein; RAN; ARA24; OK/SW-cl.81

Gene Name

IL2RA

Gene ID

3559

UniProt

P01589

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed in a variety of tissues. .

Applications

ELISA

Field of Research

Small G Proteins, Spindle

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 388 1796 95 475 1666 Standard deviation 2.77 10.90 77.41 4.15 20.71 49.81 CV (%) 3.8% 2.8% 4.3% 4.4% 4.4% 3.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 388 1796 95 475 1666 Standard deviation 2.77 10.90 77.41 4.15 20.71 49.81 CV (%) 3.8% 2.8% 4.3% 4.4% 4.4% 3.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 73 388 1796 95 475 1666 Mean (pg/ml) 73 388 1796 95 475 1666 Standard deviation 2.77 10.90 77.41 4.15 20.71 49.81 Standard deviation 2.77 10.90 77.41 4.15 20.71 49.81 CV (%) 3.8% 2.8% 4.3% 4.4% 4.4% 3.0% CV (%) 3.8% 2.8% 4.3% 4.4% 4.4% 3.0%

Sensitivity

<10 pg/mL

Function

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity) . The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30819 MW

Specificity

Natural and recombinant human IL-2R

Anticoagulant

Heparin or EDTA

Gene Name Synonym

GTP-binding nuclear protein Ran

Subcellular Location

Nucleus. Cytoplasm. Melanosome. Nucleus envelope . Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis. Colocalizes with NEMP1 at the nuclear envelope (By similarity) . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.019 0.113 0.198 0.369 0.7 1.108 1.77 2.323 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.019 0.113 0.198 0.369 0.7 1.108 1.77 2.323 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.019 0.113 0.198 0.369 0.7 1.108 1.77 2.323 O.D. 0.019 0.113 0.198 0.369 0.7 1.108 1.77 2.323