Human GASP-1 ELISA Kit PicoKine®

About this product

Human GASP-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human GASP-1 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2310

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

G-protein coupled receptor-associated sorting protein 1 is a protein that in humans is encoded by the GPRASP1 gene. The protein may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It targets receptors for degradation in lysosomes. The receptors interacting with this sorting protein include D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CB1R) . Multiple alternatively spliced transcript variants encoding the same protein have been identified.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 33 34 59 45 3.52 7.9% Sample 2 182 142 172 197 173 8.52 4.9% Sample 3 901 838 646 717 776 21.95 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 33 34 59 45 3.52 7.9% Sample 1 53 33 34 59 45 3.52 7.9% Sample 2 182 142 172 197 173 8.52 4.9% Sample 2 182 142 172 197 173 8.52 4.9% Sample 3 901 838 646 717 776 21.95 2.8% Sample 3 901 838 646 717 776 21.95 2.8%

Synonyms

GASP, GASP1, GASP-1

Gene Name

GPRASP1

Gene ID

9737

UniProt

Q5JY77

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

31.2-2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 182 901 49 214 877 Standard deviation 3.77 3.82 46.76 1.81 4.64 27.71 CV (%) 7.1% 2.1% 5.2% 3.7% 2.2% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 182 901 49 214 877 Standard deviation 3.77 3.82 46.76 1.81 4.64 27.71 CV (%) 7.1% 2.1% 5.2% 3.7% 2.2% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 182 901 49 214 877 Mean (pg/ml) 53 182 901 49 214 877 Standard deviation 3.77 3.82 46.76 1.81 4.64 27.71 Standard deviation 3.77 3.82 46.76 1.81 4.64 27.71 CV (%) 7.1% 2.1% 5.2% 3.7% 2.2% 3.2% CV (%) 7.1% 2.1% 5.2% 3.7% 2.2% 3.2%

Sensitivity

<5 pg/mL

Function

Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

156865 MW

Specificity

Natural and recombinant human GASP-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

G protein-coupled receptor associated sorting protein 1

Sequence Similarities

Belongs to the GPRASP family.

Protein Name

G-protein coupled receptor-associated sorting protein 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.061 0.137 0.2 0.318 0.567 0.976 1.511 2.059 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.061 0.137 0.2 0.318 0.567 0.976 1.511 2.059 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.061 0.137 0.2 0.318 0.567 0.976 1.511 2.059 O.D. 0.061 0.137 0.2 0.318 0.567 0.976 1.511 2.059