- Catalog number
- EK2311
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts ly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 44 33 53 41 3.25 7.9% Sample 2 218 200 197 238 213 14.35 6.7% Sample 3 601 969 744 549 716 17.97 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 44 33 53 41 3.25 7.9% Sample 1 35 44 33 53 41 3.25 7.9% Sample 2 218 200 197 238 213 14.35 6.7% Sample 2 218 200 197 238 213 14.35 6.7% Sample 3 601 969 744 549 716 17.97 2.5% Sample 3 601 969 744 549 716 17.97 2.5%
- Synonyms
- DNA repair protein XRCC2; X-ray repair cross-complementing protein 2; Xrcc2
- Gene Name
- GIT1
- Gene ID
- Q9Y2X7
- UniProt
- Q9Y2X7
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Expressed at low level in somatic tissues and at high level in testis.
- Applications
- ELISA
- Field of Research
- Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 31.2-2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2%
- Sensitivity
- <5 pg/mL
- Function
- Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 84341 MW
- Specificity
- Natural and recombinant human GIT1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- X-ray repair complementing defective repair in Chinese hamster cells 2
- Subcellular Location
- Nucleus.
- Protein Name
- DNA repair protein XRCC2
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05
