- Catalog number
- EK2318
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Huntingtin-interacting protein 1 also known as HIP-1 is a protein that in humans is encoded by the HIP1 gene. The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Sample 3 1548 1817 1980 1824 1792 52.87 3.0%
- Synonyms
- T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; CD8A; MAL
- Gene Name
- Hip1
- Gene ID
- 3092
- UniProt
- Q8VD75
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. .
- Applications
- ELISA
- Field of Research
- Neurodegenerative Disease, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 62.5-4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6%
- Sensitivity
- <10 pg/mL
- Function
- Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 115202 MW
- Specificity
- Natural and recombinant mouse Hip
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- T-cell surface glycoprotein CD8 alpha chain
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Protein Name
- Lysosome-associated membrane glycoprotein 2 (Lamp2)
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149
