- Catalog number
- EK2319
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kallikrein-15 is a protein that in humans is encoded by the KLK15 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In prostate cancer, this gene has increased expression, which indicates its possible use as a diagnostic or prognostic marker for prostate cancer. The gene contains multiple polyadenylation sites and alternative splicing results in multiple transcript variants encoding distinct isoforms.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Sample 3 1548 1817 1980 1824 1792 52.87 3.0%
- Synonyms
- ACO, HSRNASPH
- Gene Name
- KLK15
- Gene ID
- 55554
- UniProt
- Q9H2R5
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Highest expression in the thyroid gland. Also expressed in the prostate, salivary, and adrenal glands and in the colon testis and kidney.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 39.1-2,500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6%
- Sensitivity
- <10 pg/mL
- Function
- Protease whose physiological substrate is not yet known.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 28087 MW
- Specificity
- Natural and recombinant human KLK15
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kallikrein related peptidase 15
- Sequence Similarities
- Belongs to the peptidase S1 family. Kallikrein subfamily.
- Protein Name
- Kallikrein-15
- Sample Data
- Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341
