Human LAG-3 ELISA Kit PicoKine®

About this product

Human LAG-3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human LAG-3 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2320

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene. Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 116 113 133 116 7.45 6.5% Sample 2 622 580 685 388 569 41.69 7.3% Sample 3 2155 1954 2377 2266 2188 68.48 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 116 113 133 116 7.45 6.5% Sample 1 100 116 113 133 116 7.45 6.5% Sample 2 622 580 685 388 569 41.69 7.3% Sample 2 622 580 685 388 569 41.69 7.3% Sample 3 2155 1954 2377 2266 2188 68.48 3.1% Sample 3 2155 1954 2377 2266 2188 68.48 3.1%

Synonyms

CD223

Gene Name

LAG3

Gene ID

3902

UniProt

P18627

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Primarily expressed in activated T-cells and a subset of natural killer (NK) cells.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

93.8-6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 622 2155 126 601 2836 Standard deviation 2.9 16.86 93.31 9.32 24.28 109.47 CV (%) 2.9% 2.7% 4.3% 7.4% 4.0% 3.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 622 2155 126 601 2836 Standard deviation 2.9 16.86 93.31 9.32 24.28 109.47 CV (%) 2.9% 2.7% 4.3% 7.4% 4.0% 3.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 622 2155 126 601 2836 Mean (pg/ml) 100 622 2155 126 601 2836 Standard deviation 2.9 16.86 93.31 9.32 24.28 109.47 Standard deviation 2.9 16.86 93.31 9.32 24.28 109.47 CV (%) 2.9% 2.7% 4.3% 7.4% 4.0% 3.9% CV (%) 2.9% 2.7% 4.3% 7.4% 4.0% 3.9%

Sensitivity

<15 pg/mL

Function

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cellsl. Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity) . FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity) .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

57449 MW

Specificity

Natural and recombinant human LAG-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Lymphocyte activating 3

Sequence Similarities

Belongs to the LAG3 family.

Protein Name

Lymphocyte activation gene 3 protein

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.109 0.159 0.281 0.513 0.923 1.608 2.329 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.109 0.159 0.281 0.513 0.923 1.608 2.329 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.109 0.159 0.281 0.513 0.923 1.608 2.329 O.D. 0.051 0.109 0.159 0.281 0.513 0.923 1.608 2.329