- Catalog number
- EK2324
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The myosin-binding protein C, cardiac-type is a protein that in humans is encode by the MYBPC3 gene. MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 289 236 248 255 20.32 8.0% Sample 2 857 811 1169 706 886 57.04 6.4% Sample 3 2823 4400 4706 4814 4186 224.77 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 289 236 248 255 20.32 8.0% Sample 1 247 289 236 248 255 20.32 8.0% Sample 2 857 811 1169 706 886 57.04 6.4% Sample 2 857 811 1169 706 886 57.04 6.4% Sample 3 2823 4400 4706 4814 4186 224.77 5.4% Sample 3 2823 4400 4706 4814 4186 224.77 5.4%
- Synonyms
- Myosin-binding protein C, cardiac-type; Cardiac MyBP-C; C-protein, cardiac muscle isoform; MYBPC3
- Gene Name
- MYBPC3
- Gene ID
- 4607
- UniProt
- Q14896
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Red blood cells.
- Applications
- ELISA
- Field of Research
- Cardiovascular, Contractility, Hypertrophy
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 156.0-10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 857 2823 268 1023 3733 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 857 2823 268 1023 3733 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 857 2823 268 1023 3733 Mean (pg/ml) 247 857 2823 268 1023 3733 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9% CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9%
- Sensitivity
- <20 pg/mL
- Function
- Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 140762 MW
- Specificity
- Natural and recombinant human MyBPC3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Myosin binding protein C, cardiac
- Sample Data
- Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025
