- Catalog number
- EK2334
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 52 40 46 44 2.07 4.8% Sample 2 186 247 206 247 222 6.25 2.8% Sample 3 531 704 852 892 745 32.25 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 52 40 46 44 2.07 4.8% Sample 1 36 52 40 46 44 2.07 4.8% Sample 2 186 247 206 247 222 6.25 2.8% Sample 2 186 247 206 247 222 6.25 2.8% Sample 3 531 704 852 892 745 32.25 4.3% Sample 3 531 704 852 892 745 32.25 4.3%
- Synonyms
- Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; Vegfa; Vegf
- Gene Name
- VEGFA
- Gene ID
- 403802
- UniProt
- Q9MYV3
- Reactivity
- Canine
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 31.2-2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 531 46 184 505 Standard deviation 2.03 9.65 11.52 1.64 12.11 33.13 CV (%) 5.7% 5.2% 2.2% 3.6% 6.6% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 531 46 184 505 Standard deviation 2.03 9.65 11.52 1.64 12.11 33.13 CV (%) 5.7% 5.2% 2.2% 3.6% 6.6% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 531 46 184 505 Mean (pg/ml) 36 186 531 46 184 505 Standard deviation 2.03 9.65 11.52 1.64 12.11 33.13 Standard deviation 2.03 9.65 11.52 1.64 12.11 33.13 CV (%) 5.7% 5.2% 2.2% 3.6% 6.6% 6.6% CV (%) 5.7% 5.2% 2.2% 3.6% 6.6% 6.6%
- Sensitivity
- <5 pg/mL
- Function
- Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25175 MW
- Specificity
- Natural and recombinant canine VEGF
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Vascular endothelial growth factor A
- Subcellular Location
- Isoform VEGF-1: Secreted.
- Protein Name
- Vascular endothelial growth factor A
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.052 0.134 0.294 0.612 1.031 1.57 1.865 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.052 0.134 0.294 0.612 1.031 1.57 1.865 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.052 0.134 0.294 0.612 1.031 1.57 1.865 O.D. 0.007 0.052 0.134 0.294 0.612 1.031 1.57 1.865
