- Catalog number
- EK2335
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 59 60 48 55 1.19 2.2% Sample 2 136 180 221 130 167 9.95 6.0% Sample 3 514 934 883 903 809 20.62 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 59 60 48 55 1.19 2.2% Sample 1 52 59 60 48 55 1.19 2.2% Sample 2 136 180 221 130 167 9.95 6.0% Sample 2 136 180 221 130 167 9.95 6.0% Sample 3 514 934 883 903 809 20.62 2.6% Sample 3 514 934 883 903 809 20.62 2.6%
- Synonyms
- Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10
- Gene Name
- IL10
- Gene ID
- 403628
- UniProt
- P48411
- Reactivity
- Canine
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Widely expressed with highest levels in lymphoid tissues. .
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 31.2-2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1%
- Sensitivity
- <5 pg/mL
- Function
- Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 20906 MW
- Specificity
- Natural and recombinant canine IL-10
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin 10
- Subcellular Location
- Secreted.
- Protein Name
- Receptor tyrosine-protein kinase erbB-2
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217
