Canine IL-6 ELISA Kit PicoKine®

About this product

Canine IL-6 ELISA Kit PicoKine® (96 Tests) . Quantitate Canine IL-6 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2336

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, the IL6 gene is mapped to chromosome 7p15.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 103 109 81 104 5.38 5.2% Sample 2 293 461 291 458 376 19.84 5.3% Sample 3 1961 1148 1125 1507 1435 38.61 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 103 109 81 104 5.38 5.2% Sample 1 124 103 109 81 104 5.38 5.2% Sample 2 293 461 291 458 376 19.84 5.3% Sample 2 293 461 291 458 376 19.84 5.3% Sample 3 1961 1148 1125 1507 1435 38.61 2.7% Sample 3 1961 1148 1125 1507 1435 38.61 2.7%

Synonyms

Interleukin-6; IL-6; Il6; Il-6

Gene Name

IL6

Gene ID

403985

UniProt

P41323

Reactivity

Canine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed in activated macrophages (at protein level) .

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 293 1961 64 428 1382 Standard deviation 9.92 22.59 129.23 1.75 27.01 80.99 CV (%) 8.0% 7.7% 6.6% 2.7% 6.3% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 293 1961 64 428 1382 Standard deviation 9.92 22.59 129.23 1.75 27.01 80.99 CV (%) 8.0% 7.7% 6.6% 2.7% 6.3% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 124 293 1961 64 428 1382 Mean (pg/ml) 124 293 1961 64 428 1382 Standard deviation 9.92 22.59 129.23 1.75 27.01 80.99 Standard deviation 9.92 22.59 129.23 1.75 27.01 80.99 CV (%) 8.0% 7.7% 6.6% 2.7% 6.3% 5.9% CV (%) 8.0% 7.7% 6.6% 2.7% 6.3% 5.9%

Sensitivity

<10 pg/mL

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T (H) 17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity) . .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22945 MW

Specificity

Natural and recombinant canine IL-6

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 6

Subcellular Location

Secreted.

Protein Name

Fibroblast growth factor receptor 1

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.009 0.054 0.136 0.324 0.615 1.027 1.571 2.265 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.009 0.054 0.136 0.324 0.615 1.027 1.571 2.265 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.009 0.054 0.136 0.324 0.615 1.027 1.571 2.265 O.D. 0.009 0.054 0.136 0.324 0.615 1.027 1.571 2.265