Canine IL-12/IL-23 p40 ELISA Kit PicoKine®

About this product

Canine IL-12/IL-23 p40 ELISA Kit PicoKine® (96 Tests) . Quantitate Canine IL-12/IL-23 p40 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2340

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 120 106 63 117 102 4.43 4.4% Sample 2 420 314 465 281 370 25.53 6.9% Sample 3 1250 1662 1439 1781 1533 65.31 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 120 106 63 117 102 4.43 4.4% Sample 1 120 106 63 117 102 4.43 4.4% Sample 2 420 314 465 281 370 25.53 6.9% Sample 2 420 314 465 281 370 25.53 6.9% Sample 3 1250 1662 1439 1781 1533 65.31 4.3% Sample 3 1250 1662 1439 1781 1533 65.31 4.3%

Synonyms

Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; Il12b

Gene Name

IL12B

Gene ID

403976

UniProt

Q28268

Reactivity

Canine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 120 420 1250 67 439 1792 Standard deviation 2.87 15.08 67.75 2.36 33.67 40.86 CV (%) 2.4% 3.6% 5.4% 3.5% 7.7% 2.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 120 420 1250 67 439 1792 Standard deviation 2.87 15.08 67.75 2.36 33.67 40.86 CV (%) 2.4% 3.6% 5.4% 3.5% 7.7% 2.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 120 420 1250 67 439 1792 Mean (pg/ml) 120 420 1250 67 439 1792 Standard deviation 2.87 15.08 67.75 2.36 33.67 40.86 Standard deviation 2.87 15.08 67.75 2.36 33.67 40.86 CV (%) 2.4% 3.6% 5.4% 3.5% 7.7% 2.3% CV (%) 2.4% 3.6% 5.4% 3.5% 7.7% 2.3%

Sensitivity

<10 pg/mL

Function

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

37410 MW

Specificity

Natural and recombinant canine il-12

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 12b

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.06 0.095 0.134 0.198 0.322 0.598 1.084 1.858 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.06 0.095 0.134 0.198 0.322 0.598 1.084 1.858 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.06 0.095 0.134 0.198 0.322 0.598 1.084 1.858 O.D. 0.06 0.095 0.134 0.198 0.322 0.598 1.084 1.858