- Catalog number
- EK2346
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 28 27 44 36 1.96 5.4% Sample 2 143 133 149 151 144 9.60 6.7% Sample 3 567 701 609 389 567 26.68 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 28 27 44 36 1.96 5.4% Sample 1 46 28 27 44 36 1.96 5.4% Sample 2 143 133 149 151 144 9.60 6.7% Sample 2 143 133 149 151 144 9.60 6.7% Sample 3 567 701 609 389 567 26.68 4.7% Sample 3 567 701 609 389 567 26.68 4.7%
- Synonyms
- Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2
- Gene Name
- TNF
- Gene ID
- 397086
- UniProt
- P23563
- Reactivity
- Porcine
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cardiovascular, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Metabolism, Signal Transduction, TNF Superfamily, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 23.4-1,500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 143 567 28 109 477 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 143 567 28 109 477 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 143 567 28 109 477 Mean (pg/ml) 46 143 567 28 109 477 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3% CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3%
- Sensitivity
- <5 pg/mL
- Function
- Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 25254 MW
- Specificity
- Natural and recombinant porcine TNF-alpha
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor
- Subcellular Location
- Cell membrane; Single-pass type II membrane protein.
- Sequence Similarities
- Belongs to the IL-15/IL-21 family.
- Protein Name
- Tumor necrosis factor
- Sample Data
- Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487
