Porcine IL-12/IL-23 p40 ELISA Kit PicoKine®

About this product

Porcine IL-12/IL-23 p40 ELISA Kit PicoKine® (96 Tests) . Quantitate Porcine IL-12/IL-23 p40 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2353

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 68 60 58 61 3.91 6.4% Sample 2 248 320 258 257 271 14.97 5.5% Sample 3 822 1477 775 1414 1122 28.61 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 68 60 58 61 3.91 6.4% Sample 1 59 68 60 58 61 3.91 6.4% Sample 2 248 320 258 257 271 14.97 5.5% Sample 2 248 320 258 257 271 14.97 5.5% Sample 3 822 1477 775 1414 1122 28.61 2.6% Sample 3 822 1477 775 1414 1122 28.61 2.6%

Synonyms

Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; Il12b

Gene Name

IL12B

Gene ID

397076

UniProt

Q28268

Reactivity

Porcine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

46.9-3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 248 822 88 333 1444 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 248 822 88 333 1444 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 248 822 88 333 1444 Mean (pg/ml) 59 248 822 88 333 1444 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4% CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4%

Sensitivity

<5 pg/mL

Function

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

37410 MW

Specificity

Natural and recombinant porcine IL-12

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 12b

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226