Equine IL-10 ELISA Kit PicoKine®

About this product

Equine IL-10 ELISA Kit PicoKine® (96 Tests) . Quantitate Equine IL-10 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2361

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.04 3.9% Sample 2 2 2 2 2 2 0.16 8.0% Sample 3 6 7 9 6 7 0.16 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.04 3.9% Sample 1 1 1 1 1 1 0.04 3.9% Sample 2 2 2 2 2 2 0.16 8.0% Sample 2 2 2 2 2 2 0.16 8.0% Sample 3 6 7 9 6 7 0.16 2.3% Sample 3 6 7 9 6 7 0.16 2.3%

Synonyms

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Gene Name

IL10

Gene ID

100034187

UniProt

Q28374

Reactivity

Equine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Widely expressed with highest levels in lymphoid tissues. .

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

0.3-20 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 6 1 2 6 Standard deviation 0.05 0.05 0.18 0.04/td> 0.05 0.29 CV (%) 5.0% 2.7% 3.1% 4.2% 2.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 6 1 2 6 Standard deviation 0.05 0.05 0.18 0.04/td> 0.05 0.29 CV (%) 5.0% 2.7% 3.1% 4.2% 2.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 2 6 1 2 6 Mean (pg/ml) 1 2 6 1 2 6 Standard deviation 0.05 0.05 0.18 0.04/td> 0.05 0.29 Standard deviation 0.05 0.05 0.18 0.04/td> 0.05 0.29 CV (%) 5.0% 2.7% 3.1% 4.2% 2.7% 4.9% CV (%) 5.0% 2.7% 3.1% 4.2% 2.7% 4.9%

Sensitivity

<50 pg/mL

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20451 MW

Specificity

Natural and recombinant equine IL-10

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 10

Subcellular Location

Secreted.

Protein Name

Receptor tyrosine-protein kinase erbB-2

Sample Data

Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.036 0.088 0.137 0.241 0.406 0.702 1.218 1.888 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.036 0.088 0.137 0.241 0.406 0.702 1.218 1.888 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.3 0.63 1.25 2.5 5 10 20 O.D. 0.036 0.088 0.137 0.241 0.406 0.702 1.218 1.888 O.D. 0.036 0.088 0.137 0.241 0.406 0.702 1.218 1.888