- Catalog number
- EK2366
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- IL2RB (INTERLEUKIN 2 RECEPTOR, BETA), also called CD122, is a protein that in humans is encoded by the IL2RB gene. The gene represents the beta subunit and is a type I membrane protein. IL2RB is mapped to 22q12.3. The IL2RB gene contains 10 exons spanning about 24.3 kb and that the gene product is encoded by exons 2-10. The IL2 receptor is present in 3 forms with respect to binding ability to IL2: high, intermediate, and low affinity forms. Analysis of mutants of the beta chain of the IL2 receptor reveals that the granulocyte and monocyte differentiation signals are triggered by different cytoplasmic domains, showing that the signaling pathways responsible for these unique developmental outcomes are separable.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 247 170 176 205 7.06 3.4% Sample 2 967 788 648 692 774 30.41 3.9% Sample 3 2443 3274 2293 3366 2844 199.08 7.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 247 170 176 205 7.06 3.4% Sample 1 228 247 170 176 205 7.06 3.4% Sample 2 967 788 648 692 774 30.41 3.9% Sample 2 967 788 648 692 774 30.41 3.9% Sample 3 2443 3274 2293 3366 2844 199.08 7.0% Sample 3 2443 3274 2293 3366 2844 199.08 7.0%
- Synonyms
- Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; High affinity IL-2 receptor subunit beta; p70-75; CD122; Il2rb
- Gene Name
- IL2
- Gene ID
- 100034204
- UniProt
- P37997
- Reactivity
- Equine
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Expressed in lung (at protein level) . Expressed predominantly in lung, and at a much lesser extent in kidney. Also expressed in fetal lung, but not in liver, kidney and brain. .
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Lymphoid, Myeloid, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Tyrosine Kinases
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 125.0-8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 967 2443 185 821 3177 Standard deviation 8.87 67.98 54.48 7.51/td> 18.80 231.29 CV (%) 3.9% 7.0% 2.2% 4.1% 2.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 967 2443 185 821 3177 Standard deviation 8.87 67.98 54.48 7.51/td> 18.80 231.29 CV (%) 3.9% 7.0% 2.2% 4.1% 2.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 228 967 2443 185 821 3177 Mean (pg/ml) 228 967 2443 185 821 3177 Standard deviation 8.87 67.98 54.48 7.51/td> 18.80 231.29 Standard deviation 8.87 67.98 54.48 7.51/td> 18.80 231.29 CV (%) 3.9% 7.0% 2.2% 4.1% 2.3% 7.3% CV (%) 3.9% 7.0% 2.2% 4.1% 2.3% 7.3%
- Sensitivity
- <15 pg/mL
- Function
- Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17086 MW
- Specificity
- Natural and recombinant equine IL-2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-2 receptor subunit beta
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Protein Name
- Interleukin-2 receptor subunit beta
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.153 0.223 0.326 0.552 0.928 1.402 2.074 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.153 0.223 0.326 0.552 0.928 1.402 2.074 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.153 0.223 0.326 0.552 0.928 1.402 2.074 O.D. 0.052 0.153 0.223 0.326 0.552 0.928 1.402 2.074
