- Catalog number
- EK2375
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interferon gamma receptor 1 (IFNGR1), also known as CD119 (Cluster of Differentiation 119), is a protein that in humans is encoded by the IFNGR1 gene. This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 37 51 32 45 3.51 7.8% Sample 2 125 191 228 175 180 9.99 5.6% Sample 3 732 886 986 931 884 39.59 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 37 51 32 45 3.51 7.8% Sample 1 60 37 51 32 45 3.51 7.8% Sample 2 125 191 228 175 180 9.99 5.6% Sample 2 125 191 228 175 180 9.99 5.6% Sample 3 732 886 986 931 884 39.59 4.5% Sample 3 732 886 986 931 884 39.59 4.5%
- Synonyms
- Interferon gamma receptor 1; IFN-gamma receptor 1; IFN-gamma-R1; CDw119; CD119; IFNGR1
- Gene Name
- IFNG
- Gene ID
- 102128291
- UniProt
- P63309
- Reactivity
- Primate
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Liver and small intestine. Also found in ovary, testis and kidney.
- Applications
- ELISA
- Field of Research
- Antiviral Signaling, Cytokines, Hematopoietic Progenitors, Host-Virus Interaction, Immune System Diseases, Immunology, Innate Immunity, Interferons, Interspecies Interaction, Lymphoid, Macrophage/Inflammation, Microbiology, Myeloid, Stem Cells, Surface Molecules, T Lymphocytic Lineage
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 31.3-2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 125 732 36 195 553 Standard deviation 1.47 8.31 31.11 1.43 11.78 27.48 CV (%) 2.5% 6.7% 4.3% 4.0% 6.0% 5.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 125 732 36 195 553 Standard deviation 1.47 8.31 31.11 1.43 11.78 27.48 CV (%) 2.5% 6.7% 4.3% 4.0% 6.0% 5.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 125 732 36 195 553 Mean (pg/ml) 60 125 732 36 195 553 Standard deviation 1.47 8.31 31.11 1.43 11.78 27.48 Standard deviation 1.47 8.31 31.11 1.43 11.78 27.48 CV (%) 2.5% 6.7% 4.3% 4.0% 6.0% 5.0% CV (%) 2.5% 6.7% 4.3% 4.0% 6.0% 5.0%
- Sensitivity
- <5 pg/mL
- Function
- Associates with IFNGR2 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed:7615558, PubMed:2971451, PubMed:7617032, PubMed:10986460) . Ligand binding stimulates activation of the JAK/STAT signaling pathway (PubMed:7673114) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 19332 MW
- Specificity
- Natural and recombinant primate IFN-gamma
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interferon gamma receptor 1
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein .
- Protein Name
- Interferon gamma receptor 1
- Sample Data
- Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.054 0.107 0.155 0.27 0.456 0.838 1.38 2.253 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.054 0.107 0.155 0.27 0.456 0.838 1.38 2.253 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.054 0.107 0.155 0.27 0.456 0.838 1.38 2.253 O.D. 0.054 0.107 0.155 0.27 0.456 0.838 1.38 2.253
