- Catalog number
- EK2378
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS) . After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 8 12 10 10 0.62 6.1% Sample 2 33 26 37 38 34 1.14 3.4% Sample 3 131 111 190 128 140 7.01 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 8 12 10 10 0.62 6.1% Sample 1 11 8 12 10 10 0.62 6.1% Sample 2 33 26 37 38 34 1.14 3.4% Sample 2 33 26 37 38 34 1.14 3.4% Sample 3 131 111 190 128 140 7.01 5.0% Sample 3 131 111 190 128 140 7.01 5.0%
- Synonyms
- Interleukin-18; IL-18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Il18; Igif
- Gene Name
- IL18
- Gene ID
- 574151
- Reactivity
- Monkey
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Capture Antibody
- Monoclonal antibody from goat
- Detection Antibody
- Monoclonal antibody from goat
- Tissue Specificity
- Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cancer Metabolism, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA)
- Detection Range
- 6.3-400 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 33 131 7 46 128 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 33 131 7 46 128 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 33 131 7 46 128 Mean (pg/ml) 11 33 131 7 46 128 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 Standard deviation 0.34 1.83 6.71 0.24 2.10 6.53 CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1% CV (%) 3.1% 5.5% 5.1% 3.4% 4.6% 5.1%
- Sensitivity
- <1 pg/mL
- Function
- Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant monkey IL-18
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-18
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the IL-15/IL-21 family.
- Protein Name
- Interleukin-18
- Sample Data
- Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 Concentration (pg/ml) 0 6.3 12.5 25 50 100 200 400 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2 O.D. 0.045 0.106 0.157 0.285 0.508 0.858 1.587 2.2
