Anti-PHD2/EGLN1 Antibody Picoband®

About this product

Boster Bio Anti-PHD2/EGLN1 Antibody Picoband® catalog # A00415-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00415-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3) .

Synonyms

EGLN1; C1orf12; PNAS-118; PNAS-137; Egl nine homolog 1; EC 1.14.11.29; Hypoxia-inducible factor prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2; Prolyl hydroxylase domain-containing protein 2; PHD2; SM-20

Gene Name

EGLN1

Gene ID

54583

UniProt

Q9GZT9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PHD2/EGLN1. Human PHD2/EGLN1 shares 100% amino acid (aa) sequence identity with both mouse and rat PHD2/EGLN1.

Clonality

Polyclonal

Applications

WB, IP, Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

46 kDa

Observed Molecular Weight

46 kDa

Applications Notes

Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human<br> Immunoprecipitation, 0.5-2 μg/mL, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br>

Gene Name Synonym

Egl-9 family hypoxia inducible factor 1

Protein Name

Egl nine homolog 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.