Anti-TNF-Alpha (Q178) Antibody

About this product

Boster Bio Anti-TNF-Alpha (Q178) Antibody catalog # A00002. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00002

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; TNF; TNFA, TNFSF2

Gene Name

TNF

UniProt

P01375

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha.

Clonality

Polyclonal

Applications

WB, IHC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208) . Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

25644 MW

Gene Name Synonym

Tumor necrosis factor

Subcellular Location

Cell membrane

Protein Name

Tumor necrosis factor

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2