Anti-ErbB2/HER2 (phospho-Y1221/Y1222) Antibody

About this product

Boster Bio Anti-ErbB2/HER2 (phospho-Y1221/Y1222) Antibody catalog # A00010Y1221Y1222. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00010Y1221Y1222

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Receptor tyrosine-protein kinase erbB-2;2.7.10.1; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2; HER2, MLN19, NEU, NGL

Gene Name

ERBB2

UniProt

P04626

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human ErbB2/HER2 around the phosphorylation site of Tyrosine 1221/1222.

Clonality

Polyclonal

Tissue Specificity

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Applications

IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs) . Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

137910 MW

Applications Notes

IHC: 1:50-1:200

Gene Name Synonym

Receptor tyrosine-protein kinase erbB-2

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.

Protein Name

Receptor tyrosine-protein kinase erbB-2

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2