Anti-PI3K p110 Alpha (R519) PIK3CA Antibody

About this product

Boster Bio Anti-PI3K p110 Alpha (R519) PIK3CA Antibody catalog # A00029. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00029

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha;2.7.1.153

Gene Name

PIK3CA

UniProt

P42336

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 500-550 of Human PI3K p110α.

Clonality

Polyclonal

Applications

IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

124284 MW

Applications Notes

IHC: 1:50-1:200

Gene Name Synonym

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Protein Name

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2