Anti-Rb (P789) RB1 Antibody

Catalog number A00039

Price for A00039

Size / count 100 µL

About this product

Boster Bio Anti-Rb (P789) RB1 Antibody catalog # A00039. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number: A00039
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
Synonyms: Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1
Gene Name: RB1
UniProt: P06400
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide, corresponding to amino acids 761-810 of Human Rb.
Clonality: Polyclonal
Tissue Specificity: Expressed in the retina.
Applications: WB
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration: 1 mg/mL
Form: Liquid
Function: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity) .
Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight: 106159 MW
Applications Notes: WB: 1:500-1:1000
Gene Name Synonym: Retinoblastoma-associated protein
Subcellular Location: Nucleus
Protein Name: Retinoblastoma-associated protein
Contents: Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Frequently Asked Questions

What is the catalog number for Anti-Rb (P789) RB1 Antibody?

The catalog number for Anti-Rb (P789) RB1 Antibody is A00039.

What sizes are available for Anti-Rb (P789) RB1 Antibody?

Anti-Rb (P789) RB1 Antibody is available in the following size(s): 100 µL.

Is Anti-Rb (P789) RB1 Antibody in stock?

Anti-Rb (P789) RB1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-Rb (P789) RB1 Antibody?

The safety data sheet for Anti-Rb (P789) RB1 Antibody is available here: https://www.bosterbio.com/msds?sku=A00039.

What is Anti-Rb (P789) RB1 Antibody used for?

Boster Bio Anti-Rb (P789) RB1 Antibody catalog # A00039. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.