Anti-Rb (phospho-S780) RB1 Antibody

About this product

Boster Bio Anti-Rb (phospho-S780) RB1 Antibody catalog # A00039S780. Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00039S780

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1

Gene Name

RB1

UniProt

P06400

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Rb around the phosphorylation site of Serine 780.

Clonality

Polyclonal

Tissue Specificity

Expressed in the retina.

Applications

IHC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

106159 MW

Gene Name Synonym

Retinoblastoma-associated protein

Subcellular Location

Nucleus

Protein Name

Retinoblastoma-associated protein

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2