Anti-Estrogen receptor ESR1 Antibody

About this product

Boster Bio Anti-Estrogen receptor ESR1 Antibody (Catalog # A00057) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00057

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1) . Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2) . Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3) .

Synonyms

ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER

Gene Name

ESR1

UniProt

P03372

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

ESR1 antibody is human, mouse and rat reactive.

Immunogen

ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1. The immunogen is located within amino acids 250 - 300 of ESR1.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Signal Transduction

Purification

ESR1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

Storage Conditions

ESR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

66216 MW

Gene Name Synonym

Estrogen receptor 1

Subcellular Location

Isoform 1: Nucleus

Prediction Reactivity

Bovine, Pig, Horse

Protein Name

Estrogen receptor 1

Isotype

IgG

Contents

ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.