Anti-Smad3 (phospho-T179) Antibody

Catalog number A00059T179

Price for A00059T179

Size / count 100 µL

About this product

Boster Bio Anti-Smad3 (phospho-T179) Antibody catalog # A00059T179. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number: A00059T179
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
Synonyms: Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
Gene Name: SMAD3
UniProt: P84022
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Threonine 179.
Clonality: Polyclonal
Applications: WB
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Concentration: 1 mg/mL
Form: Liquid
Function: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight: 48081 MW
Applications Notes: WB: 1:1000-1:2000
Gene Name Synonym: Mothers against decapentaplegic homolog 3
Subcellular Location: Cytoplasm
Protein Name: Mothers against decapentaplegic homolog 3
Contents: Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Frequently Asked Questions

What is the catalog number for Anti-Smad3 (phospho-T179) Antibody?

The catalog number for Anti-Smad3 (phospho-T179) Antibody is A00059T179.

What sizes are available for Anti-Smad3 (phospho-T179) Antibody?

Anti-Smad3 (phospho-T179) Antibody is available in the following size(s): 100 µL.

Is Anti-Smad3 (phospho-T179) Antibody in stock?

Anti-Smad3 (phospho-T179) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-Smad3 (phospho-T179) Antibody?

The safety data sheet for Anti-Smad3 (phospho-T179) Antibody is available here: https://www.bosterbio.com/msds?sku=A00059T179.

What is Anti-Smad3 (phospho-T179) Antibody used for?

Boster Bio Anti-Smad3 (phospho-T179) Antibody catalog # A00059T179. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.