Anti-FoxO1/3/4 (phospho-T24/32) Antibody

About this product

Boster Bio Anti-FoxO1/3/4 (phospho-T24/32) Antibody catalog # A00073T24. Tested in WB, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

A00073T24

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1; FKHR, FOXO1A

Gene Name

FOXO1

UniProt

Q12778/O43524/P98177

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Threonine 24.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide- damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

69662 MW

Gene Name Synonym

Forkhead box protein O1

Subcellular Location

Cytoplasm

Protein Name

Forkhead box protein O1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2