Anti-RNase III Drosha Antibody

About this product

Boster Bio Anti-RNase III Drosha Antibody catalog # A00111. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A00111

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ribonuclease 3;3.1.26.3 ; Protein Drosha ; Ribonuclease III; RNase III; p241; DROSHA; RN3, RNASE3L, RNASEN

Gene Name

DROSHA

UniProt

Q9NRR4

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human RNase III Drosha. AA range:774-823

Tissue Specificity

Ubiquitous. .

Applications

WB, IHC, IF, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

159316 MW

Gene Name Synonym

Ribonuclease 3

Subcellular Location

Nucleus . Nucleus, nucleolus . A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. .

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.