Anti-IKK Beta (phospho-Y199) IKBKB Antibody

About this product

Boster Bio Anti-IKK Beta (phospho-Y199) IKBKB Antibody catalog # A00118Y199. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00118Y199

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB;2.7.11.10; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB; IKBKB; IKKB

Gene Name

IKBKB

UniProt

O14920

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human eEF2K around the phosphorylation site of Tyrosine 199.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

Applications

IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF- dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

86564 MW

Applications Notes

IHC: 1:50-1:200

Gene Name Synonym

Inhibitor of nuclear factor kappa-B kinase subunit beta

Subcellular Location

Cytoplasm

Protein Name

Inhibitor of nuclear factor kappa-B kinase subunit beta

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2