Anti-PARP PARP1 Antibody

About this product

Boster Bio Anti-PARP PARP1 Antibody catalog # A00122. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00122

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Poly [ADP-ribose] polymerase 1; PARP-1;2.4.2.30

Gene Name

PARP1

UniProt

P09874

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human PARP1 (NP_001609.2) .

Clonality

Polyclonal

Applications

WB, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl) ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272) . Mediates the poly (ADP-ribosyl) ation of APLF and CHFR (PubMed:17396150) . Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976) . Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272) . PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272) . Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:28190768) . Mediates the poly (ADP-ribosyl) ation of histones in a HPF1-dependent manner (PubMed:27067600) . Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257) . Nuclear ATP generation is required for extensive chromatin remodeling events that are energy- consuming (PubMed:27257257) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

113084 MW

Gene Name Synonym

Poly [ADP-ribose] polymerase 1

Subcellular Location

Nucleus

Protein Name

Poly [ADP-ribose] polymerase 1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2