Anti-BRD4, HUNK1 Antibody

About this product

Boster Bio Anti-BRD4, HUNK1 Antibody (Catalog# A00123) . Tested in WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

A00123

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Bromodomain-containing protein 4; Protein HUNK1; BRD4; HUNK1

Gene Name

BRD4

UniProt

O60885

Host

Rabbit

Reactivity

Human

Immunogen

A partial length recombinant BRD4 protein (amino acids 140-500)

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB

Field of Research

Cancer, Chromatin Binding Proteins, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Transcription, Tumor Suppressors

Purification

Protein A Chromatography

Concentration

0.5-1mg/mL, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028) . In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. .

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Observed Molecular Weight

152219 MW

Gene Name Synonym

Bromodomain-containing protein 4

Subcellular Location

Nucleus. Chromosome. Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment.

Protein Name

Bromodomain-containing protein 4

Isotype

Rabbit IgG

Contents

100 μ g in 200 μ l PBS containing 0.05% BSA and 0.05% sodium azide.