Product details

Anti-MLH1 Antibody

Catalog number A00126

Price for A00126

Size / count 100 µL

About this product

Boster Bio Anti-MLH1 Antibody catalog # A00126. Tested in WB, ICC/IF, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number: A00126
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
Synonyms: DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1; COCA2
Gene Name: MLH1
UniProt: P40692
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein
Clonality: Polyclonal
Tissue Specificity: Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
Applications: WB, ICC, IF, IHC, IP, Flow Cytometry
Purification: ProA affinity purified
Concentration: 0.5-1mg/mL, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form: Liquid
Function: Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR) . DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight: 84601 MW
Gene Name Synonym: DNA mismatch repair protein Mlh1
Subcellular Location: Nucleus
Protein Name: DNA mismatch repair protein Mlh1
Contents: Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Frequently Asked Questions

What is the catalog number for Anti-MLH1 Antibody?

The catalog number for Anti-MLH1 Antibody is A00126.

What sizes are available for Anti-MLH1 Antibody?

Anti-MLH1 Antibody is available in the following size(s): 100 µL.

Is Anti-MLH1 Antibody in stock?

Anti-MLH1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Anti-MLH1 Antibody?

The safety data sheet for Anti-MLH1 Antibody is available here: https://www.bosterbio.com/msds?sku=A00126.

What is Anti-MLH1 Antibody used for?

Boster Bio Anti-MLH1 Antibody catalog # A00126. Tested in WB, ICC/IF, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.