Anti-MSH2 Antibody

About this product

Boster Bio Anti-MSH2 Antibody catalog # A00140. Tested in IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00140

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2

Gene Name

MSH2

UniProt

P43246

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human MSH2. AA range:541-590

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

IHC, IF, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Form

Liquid

Function

Component of the post-replicative DNA mismatch repair system (MMR) . Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

104743 MW

Gene Name Synonym

DNA mismatch repair protein Msh2

Subcellular Location

Nucleus .

Protein Name

DNA mismatch repair protein Msh2

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.